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KMID : 0894520090130040271
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Development & Reproduction
2009 Volume.13 No. 4 p.271 ~ p.280
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New Isolation Technique and Culture System for Clinical Applications of Human Amniotic Epithelial Stem Cells
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Woo Sang-Kyu
Jo Jung-Woun
Shin Il-Seob
Kang Sung-Keun
Ra Jeong-Chan
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Abstract
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Human placenta is abundant source of adult stem cells. Especially, amniotic epithelial cells have stem cell characteristics, expressing surface markers normally present on embryonic stem cells and germ cells. However, culturing and expanding amniotic epithelial cells in vitro without feeder cells are difficult due to endogenous characteristics of epithelial cells. In the present study, amniotic epithelial cells are isolated and proliferated in several passages by applying dithiothreitol and a Rho-associated kinase inhibitor in culture media. The cultured amniotic epithelial cells showed the epithelial and stem cell characteristics. In conclusion, human placenta-derived amniotic epithelial stem cells can be a major source of stem cells for medical treatment of various diseases without any controversial issues.
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KEYWORD
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Amniotic epithelial stem cells, Regenerative medicine, DTT, ROCK inhibitor, Stem cell therapy
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